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  • The Influence of Micronutrients in Cell Culture: A ...

    2013-5-27u2002·u2002In many common culture media, the sole source of micronutrients is fetal bovine serum (FBS), which contributes to only 5–10% of the media composition. Minimal attention has been dedicated to FBS composition, micronutrients in cell cultures as a whole, or the influence of micronutrients on the viability and genetics of cultured cells.

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  • Direct comparison of distinct naive pluripotent states in ...

    2017-4-21u2002·u2002On day 7, EBs were plated in NIM containing 5% fetal bovine serum to promote attachment for 8 h and were refreshed gently with fresh NIM once the EBs were attached. Neural rosettes were observed ...

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  • The Silk-protein Sericin Induces Rapid Melanization of ...

    2016-3-4u2002·u2002Photomicrographs (a–f) show human retinal pigment epithelial cells cultured for 12 days with or without 1% sericin or 10% fetal bovine serum (FBS) using two different basal media.

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  • Animal Product Alternatives

    2022-1-4u2002·u2002So roughly 0.5 L of serum is needed to make 1 kg of meat. A single cow fetus can produce 150-550 ml of serum, depending on its age. Source: 'A bovine fetus of 3 months yields about 150 ml of raw FBS, at 6 months 350 ml and at 9 months (near-term) 550 ml.' Jochems et al., The use of fetal bovine serum: ethical or scientific problem?. Thus 1 ...

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  • PRAF2 overexpression predicts poor prognosis and

    2019-6-14u2002·u2002For colony formation assay, a total of 200 cells were placed in a fresh 6-well plate and cultured in medium containing 10% fetal bovine serum, with medium replacement every 3–4 days. After 2 weeks, cells were fixed with 4% paraformaldehyde and stained with 0.1% crystal violet.

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  • Automated High-Throughput Method for the Fast, Robust,

    2021-12-3u2002·u2002A high-throughput method was developed for the automated enrichment of newly synthesized proteins (NSPs), which are labeled metabolically by substituting methionine with the 'click-able' analogue azidohomoalanine (AHA). A suitable conjugate containing a dibenzocyclooctyne (DBCO) group allows the specific selection of NSPs by a fast 1 h click …

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  • Protocol for the Generation of Definitive Hematopoietic ...

    2020-12-18u2002·u2002This protocol offers a detailed procedure for the in vitro differentiation of human pluripotent stem cells (hPSCs) to multipotent hematopoietic progenitors that arise from SOX17 + hemogenic endothelium, mimicking intra-embryonic, HOXA-positive, aorta-gonad mesonephros (AGM) hematopoiesis. The generated endothelium displays transcriptional similarities to cells …

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  • Tandem Paired Nicking Promotes Precise Genome Editing

    2020-1-28u2002·u2002HeLa, 293T, and MCF10A were derived from female human cells, while HCT116, DLD-1, MeT-5A, PL5, BEAS-2B, and TIGE-9 were from male human cells. All of these cell lines and their derivatives were maintained at 37°C with 5% CO 2 in a humidified incubator (PHCbi, Tokyo, Japan) except for TIGE-9 which was maintained with 3% CO 2 .

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  • (PDF) Guidance on Good Cell Culture Practice

    2021-12-29u2002·u2002Based on review by a broad range of experts and. organisations, the aim of this Guidance is to foster. consensus among all concerned with the use of cell. and tissue culture systems, in order to ...

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    Take A Sneak Peak At The Movies Coming Out This Week (8/12) Minneapolis-St. Paul Movie Theaters: A Complete Guide; Best Romantic Christmas Movies to Watch

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  • Animal Product Alternatives

    2022-1-4u2002·u2002So roughly 0.5 L of serum is needed to make 1 kg of meat. A single cow fetus can produce 150-550 ml of serum, depending on its age. Source: 'A bovine fetus of 3 months yields about 150 ml of raw FBS, at 6 months 350 ml and at 9 months (near-term) 550 ml.' Jochems et al., The use of fetal bovine serum: ethical or scientific problem?. Thus 1 ...

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  • Bisphenol F and bisphenol S promote lipid accumulation

    2021-6-1u2002·u2002BPF and BPS treatments affected hASCs differentiation, enhancing adipogenic effects. At day 14 of adipocyte differentiation, the results showed a dose-dependent increase in both BPF and BPS treatments (Fig. 1A–B).PPARγ mRNA expression was significantly increased with BPF treatments at 10 μM and 25 μM (1.72- and 2.06-fold, respectively), and with BPS at 1 μM, 10 …

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  • The Silk-protein Sericin Induces Rapid Melanization of ...

    2016-3-4u2002·u2002Photomicrographs (a–f) show human retinal pigment epithelial cells cultured for 12 days with or without 1% sericin or 10% fetal bovine serum (FBS) using two different basal media.

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  • ZFX promotes tumorigenesis and confers chemotherapy ...

    2021-9-1u2002·u2002Human KYSE30 and TE1 cells were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cells were cultivated in DMEM medium supplemented with 10% fetal bovine serum (Gibco, USA) within a humidified atmosphere containing at 37 °C in 5% CO 2. Cell transfection was performed using Lipofectamine 2000 Reagent (Invitrogen ...

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  • PRAF2 overexpression predicts poor prognosis and

    2019-6-14u2002·u2002For colony formation assay, a total of 200 cells were placed in a fresh 6-well plate and cultured in medium containing 10% fetal bovine serum, with medium replacement every 3–4 days. After 2 weeks, cells were fixed with 4% paraformaldehyde and stained with 0.1% crystal violet.

    Get Price
  • Human tendon-derived cell sheets created by magnetic

    2021-9-1u2002·u2002Minced samples were collected into a 50 mL tube, already containing an enzymatic solution of collagenase (0.1%, Sigma-Aldrich, C6885, USA) with 2 M CaCl2 (1:1000, VWR, Germany) and 1% bovine serum albumin (BSA) (Sigma-Aldrich, USA) for 1 h at 37°C under constant agitation. A ratio of 1:1 of minced tissue to the enzymatic solution was considered.

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  • Targeted expression profiling reveals distinct stages of ...

    2020-12-9u2002·u2002Adult or fetal canine fibroblasts were maintained in high-glucose DMEM supplemented with 10% Gibco™ qualified, Canada-origin fetal bovine serum (FBS), 2 mM GlutaMax, 1X non-essential amino acids, and 0.1 mM 2-mercaptoethanol. MEF monolayer preparation and culture of canine ESCs were conducted as previously described [44, 45].

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  • Gibco Cell Culture Media

    Gibco Human Plasma-like Medium (HPLM) is a new formulation designed to resemble the natural cellular environment found in the body, mimicking the metabolic profile of human plasma. Physiologically relevant - designed to resemble the natural cellular environment found in the body formulated with more than 60 polar metabolites and salt concentrations

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    2021-1-4u2002·u2002Serum proteomic analysis was performed using the aptamer-based SOMAscan 1.3k Assay (SomaLogic). SomaLogic detects 1,305 protein analytes and is optimized for analysis of human serum 59. Briefly ...

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    2016-2-29u2002·u2002Staphylococcus aureus is known as a frequent colonizer of the skin and mucosa. Among bacterial factors involved in colonization are adhesins such as the microbial surface components recognizing ...

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  • Communicable Diseases

    Sanitation, food control, vaccines, and antibiotics have reduced the toll of communicable diseases, saving millions of lives. Smallpox was eradicated in 1977, and poliomyelitis eradication is close. Measles mortality has reduced drastically yet outbreaks ...

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    Biomaterials are used to engineer functional restoration of different tissues to improve human health and the quality of life. Biomaterials can be natural or synthetic. Additive manufacturing (AM) is a novel materials processing approach to create parts or prototypes layer-by-layer directly from a computer aided design (CAD) file.

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    Others In a scientific study, when people took FARXIGA with metformin they lost an average of 6.6 pounds versus 2 pounds with metformin alone at 12 weeks. And it also helped lower their systolic blood pressure. Buy Sugar Control herbal Medicine For Diabetes Control Pack Of 2 online at a discounted price the 1 last update 29 Dec 2021 from ShopClues.com. Shop Daily Needs and …

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    2011-3-1u2002·u2002Maternal health status was assessed by means of the Medical Outcomes Study 36-Item Short-Form General Health Survey (SF-36), which assesses eight aspects of health status: general and mental health, physical and social functioning, physical and emotional role, pain, and vitality; scores on each scale can range from 0 (worst) to 100 (best). 23 ...

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    high glucose levels 104 ????tools. With respect to curative therapy for type 1 diabetes, research on pancreas or islet cell transplantation is underway but has been limited by organ availability and the risks associated with immunosuppression.

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    1 day agou2002·u2002Communities in Manitoba. Community Documents Find community resource documents to facilitate municipal administration, public works, recreation and wellness, environmental services, protective services, community development, land-use planning, community planning, and infrastructure development.

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  • Animal Product Alternatives

    2022-1-4u2002·u2002So roughly 0.5 L of serum is needed to make 1 kg of meat. A single cow fetus can produce 150-550 ml of serum, depending on its age. Source: 'A bovine fetus of 3 months yields about 150 ml of raw FBS, at 6 months 350 ml and at 9 months (near-term) 550 ml.' Jochems et al., The use of fetal bovine serum: ethical or scientific problem?. Thus 1 ...

    Get Price
  • Annexin A1–dependent tethering promotes extracellular ...

    Primary human coronary artery SMCs were obtained from Promocell (Heidelberg, Germany) and expanded in SMC growth medium 2 (Promocell) supplemented with epidermal growth factor (0.5 ng/ml), insulin (5 μg/ml), basic fibroblast growth factor-B (2 ng/ml), and 5% fetal bovine serum (FBS). Primary human VICs were obtained from human aortic valve ...

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  • Norepinephrine promotes triglyceride storage in ...

    2021-1-23u2002·u2002Norepinephrine promotes triglyceride storage in macrophages via beta2-adrenergic receptor activation ... Division of Human Nutrition and Health, Wageningen University, Wageningen, the Netherlands. Department of Internal Medicine, Radboud University Medical Center, Nijmegen, the Netherlands ... (note that all treatments were performed in the ...

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  • Platelet concentrate in bovine reproduction: effects on in ...

    2015-6-19u2002·u2002Platelet collection efficiency and product sterility test. The platelet collection efficiency for the PCs was 10.9 ± 2.3 %, indicating that 10.9 ± 2.3 ml of PC at the concentration of 1x10 9 platelet/ml were obtained from 100 ml of blood.. The bacterial sterility test performed on the PC produced in the laboratory has always given negative results, confirming that the …

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  • Progesterone, via yes‐associated protein, promotes ...

    2020-10-12u2002·u2002After that, cells in the suspension were plated and cultured in DMEM with 10% foetal bovine serum at 37°C in atmosphere with 5% CO 2 for 24 hours. Then, progesterone at various concentrations (10 −9 -10 −4 M) with or without the progesterone blocker RU486 (10 −6 M) was added to the medium to incubate for 24 hours, and then, the cells ...

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  • Human dental pulp stem cell responses to ... - BMC Oral Health

    2021-4-26u2002·u2002Direct pulp capping is a vital pulp therapy for a pin-point dental pulp exposure. Applying a pulp capping material leads to the formation of a dentin bridge and protects pulp vitality. The aim of this study was to compare the effects of four dental materials, DyCal®, ProRoot® MTA, Biodentine™, and TheraCal™ LC in vitro. Human dental pulp stem cells (hDPs) were isolated and …

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  • Targeted expression profiling reveals distinct stages of ...

    2020-12-9u2002·u2002Adult or fetal canine fibroblasts were maintained in high-glucose DMEM supplemented with 10% Gibco™ qualified, Canada-origin fetal bovine serum (FBS), 2 mM GlutaMax, 1X non-essential amino acids, and 0.1 mM 2-mercaptoethanol. MEF monolayer preparation and culture of canine ESCs were conducted as previously described [44, 45].

    Get Price
  • Cell Culture Cocktails with Fewer Hangovers

    2017-1-31u2002·u2002Cell Culture Cocktails with Fewer Hangovers. February 1, 2017 (Vol. 37, No. 3) The global market for cell culture media, sera, and reagents is climbing from a …

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  • Technical Manual, 20th Edition: Methods and Appendices

    2020-8-7u2002·u2002Some manufacturers recommend performing tests with whole blood; others recommend that red cells be suspended in autologous serum, plasma, or saline, or washed and resuspended in saline. Equipment Dispensers (optional): Semiautomated devices are available for dispensing equal volumes to a row of wells.

    Get Price
  • Glial Cell Lines: An Overview

    2010-12-3u2002·u2002The importance and essential functions of glial cells in the nervous system are now beginning to be understood and appreciated. Glial cell lines have been instrumental in the elucidation of many of these properties. In this Overview, the origin and properties of most of the existing cell lines for the major glial types: oligodendroglia, astroglia, microglia and Schwann …

    Get Price
  • Animal Product Alternatives

    2022-1-4u2002·u2002So roughly 0.5 L of serum is needed to make 1 kg of meat. A single cow fetus can produce 150-550 ml of serum, depending on its age. Source: 'A bovine fetus of 3 months yields about 150 ml of raw FBS, at 6 months 350 ml and at 9 months (near-term) 550 ml.' Jochems et al., The use of fetal bovine serum: ethical or scientific problem?. Thus 1 ...

    Get Price
  • Annexin A1–dependent tethering promotes extracellular ...

    Primary human coronary artery SMCs were obtained from Promocell (Heidelberg, Germany) and expanded in SMC growth medium 2 (Promocell) supplemented with epidermal growth factor (0.5 ng/ml), insulin (5 μg/ml), basic fibroblast growth factor-B (2 ng/ml), and 5% fetal bovine serum (FBS). Primary human VICs were obtained from human aortic valve ...

    Get Price
  • Norepinephrine promotes triglyceride storage in ...

    2021-1-23u2002·u2002Norepinephrine promotes triglyceride storage in macrophages via beta2-adrenergic receptor activation ... Division of Human Nutrition and Health, Wageningen University, Wageningen, the Netherlands. Department of Internal Medicine, Radboud University Medical Center, Nijmegen, the Netherlands ... (note that all treatments were performed in the ...

    Get Price
  • Platelet concentrate in bovine reproduction: effects on in ...

    2015-6-19u2002·u2002Platelet collection efficiency and product sterility test. The platelet collection efficiency for the PCs was 10.9 ± 2.3 %, indicating that 10.9 ± 2.3 ml of PC at the concentration of 1x10 9 platelet/ml were obtained from 100 ml of blood.. The bacterial sterility test performed on the PC produced in the laboratory has always given negative results, confirming that the …

    Get Price
  • Progesterone, via yes‐associated protein, promotes ...

    2020-10-12u2002·u2002After that, cells in the suspension were plated and cultured in DMEM with 10% foetal bovine serum at 37°C in atmosphere with 5% CO 2 for 24 hours. Then, progesterone at various concentrations (10 −9 -10 −4 M) with or without the progesterone blocker RU486 (10 −6 M) was added to the medium to incubate for 24 hours, and then, the cells ...

    Get Price
  • Human dental pulp stem cell responses to ... - BMC Oral

    2021-4-26u2002·u2002Direct pulp capping is a vital pulp therapy for a pin-point dental pulp exposure. Applying a pulp capping material leads to the formation of a dentin bridge and protects pulp vitality. The aim of this study was to compare the effects of four dental materials, DyCal®, ProRoot® MTA, Biodentine™, and TheraCal™ LC in vitro. Human dental pulp stem cells (hDPs) …

    Get Price
  • Targeted expression profiling reveals distinct stages of ...

    2020-12-9u2002·u2002Adult or fetal canine fibroblasts were maintained in high-glucose DMEM supplemented with 10% Gibco™ qualified, Canada-origin fetal bovine serum (FBS), 2 mM GlutaMax, 1X non-essential amino acids, and 0.1 mM 2-mercaptoethanol. MEF monolayer preparation and culture of canine ESCs were conducted as previously described [44, 45].

    Get Price
  • Cell Culture Cocktails with Fewer Hangovers

    2017-1-31u2002·u2002Cell Culture Cocktails with Fewer Hangovers. February 1, 2017 (Vol. 37, No. 3) The global market for cell culture media, sera, and reagents is climbing from a …

    Get Price
  • Technical Manual, 20th Edition: Methods and Appendices

    2020-8-7u2002·u2002Some manufacturers recommend performing tests with whole blood; others recommend that red cells be suspended in autologous serum, plasma, or saline, or washed and resuspended in saline. Equipment Dispensers (optional): Semiautomated devices are available for dispensing equal volumes to a row of wells.

    Get Price
  • Glial Cell Lines: An Overview

    2010-12-3u2002·u2002The importance and essential functions of glial cells in the nervous system are now beginning to be understood and appreciated. Glial cell lines have been instrumental in the elucidation of many of these properties. In this Overview, the origin and properties of most of the existing cell lines for the major glial types: oligodendroglia, astroglia, microglia and Schwann …

    Get Price